Abstract
EXTRACTION AND EVALUATION OF OIL FROM THE SEEDS OF HARUNGANA MADAGASCARIENSIS AND CYPERUS ESCULENTUS

Ordu J. I.* and Okafo S. E.

ABSTRACT

The study is aimed at the extraction and evaluation of oil from the seeds of Harungana madagascariensis and Cyperus esculentus. Soxhlet extraction was adopted using n- hexane, then oil extracts were analyzed for proximate and elemental composition, antimicrobial and physicochemical properties. The antimicrobial screening was carried out on five pathogenic organisms including fungi and bacteria identified as: C.albicans, K.pneumoniae, S.aureus, Escherichia coli, P.aeruginosa and aimed at determining the inhibitory activity of the extracts against the organisms. The percentage yield of the oil extracts were 31.72% and 18.91% for C.esculentus and H.madagascariensis respectively while the proximate principle revealed presence of moisture at 45.23% and 12.5%, CHO 16.0% and 35.05%, protein 6.0% and 5.2% and 1.0% and 2.5% ash content for C.esculentus and H.madagascariensis respectively. The elemental analysis showed the presence of minerals in the order K>Mg>Ca>Zn for both oil extracts. Chemical characterization of the oils showed acid value of 1.309 and 1.663mgKOH/g, peroxide value as 15.20 and 13.02 meq/KOH, saponification value of 143.10 and 190.74 mg/KOH/g, Iodine value of 80.37 and 83. 05 mg/100g and free fatty acid content of 65.78±3.52mg-g% and 70.11±1.23 mg-g% respectively for C.esculentus and H.madagascariensis. The results obtained indicates the oils extracts to have emollient and antimicrobial activities hence could be useful as potential ingredient in pharmaceutical topical formulations.

Keywords: C.esculentus, H. madagascariensis, seeds, extraction, evaluation, oil.


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