European Journal of Biomedical and Pharmaceutical Sciences

SCREENING OF ANTIBIOTIC (STAUROSPORINE) PRODUCING ACTINOBACTERIA (Streptomyces sp.) FROM TERRESTRIAL ENVIRONMENT SOIL OF THANJAVUR DISTRICT, TAMILNADU, INDIA

?Cholarajan A. and Vijayakumar R.

ABSTRACT

Actinobacteria have gained prominence in recent years because of their potential for producing antibiotics which are not only used in pharmaceutical industries but also in the agriculture practice to inhibit the growth of several phyto-pathogens. A total of 5 different types of actinobacteria were isolated from the soil of terrestrial environment from Thanjavur District. All the isolated actinobacteria were characterized and identified based on the morphological, biochemical and molecular characteristics. The isolates were belonged to the genera Streptomyces KV1, KV2, KV3, KV4 and KV5. The isolated strains were screened by cross streak and well diffusion methods. Only Streptomyces sp KV2 was selected for further studies. The result of the molecular characteristics selected Streptomyces sp. (KV2) identified as S. champavatii (KV2). The result of the screening revealed that the crude extract was effective against human and plant pathogens. The antimicrobial efficacies and solubility of the antimicrobial compound was evaluated and extracted with organic solvents. Extracted antimicrobial compound of the Streptomyces sp. KV2 was characterized by using UV, FT-IR and 1H and 13C NMR spectral analyses. Rf value in the TLC chromatogram characterized that, the metabolite comes under , -O-H, -C-O-CH3, F-CH3, and group very much related to Staurosporine.

Keywords: Terrestrial soil; Streptomyces sp. KV2; antimicrobial activity; UV; FT-IR; NMR analyses.