European Journal of Biomedical and Pharmaceutical Sciences

FIRST ORDER DERIVATIVE SPECTROSCOPIC METHOD DEVELOPMENT AND VALIDATION FOR THE SIMULTANEOUS ESTIMATION OF HYDROCHLOROTHIAZIDE AND FIMSARTAN IN SYNTHETIC MIXTURE

*Dr. Suresh Kumar and Nirma Chavda

ABSTRACT

A simple, economical, accurate, precise and less time-consuming First order derivative UV Spectrophotometric
method has been developed and validated for simultaneous estimation of Hydrochlorothiazide and Fimasartan in
synthetic mixture. In this method Hydrochlorothiazide and Fimasartan are exhibits maximum absorbance (λ max)
at 263 nm and 246 nm with methanol as the solvent. The method was validated as per the International Conference
on Harmonization (ICHQ2R1) guidelines. Drugs followed the linearity in the concentration range of 6.25-31.25
μg/mL and 30-150 μg/mL with correlation coefficient (r2) of 0.9988 and 0.9988 for Hydrochlorothiazide and
Fimasartan respectively. The validity of the proposed method was assessed by applying the standard addition
technique where the percentage recovery of the added standard was found to be 98.93 and 100.05 for
Hydrochlorothiazide and Fimasartan. The limit of detection and quantification were calculated and found to be
0.1045 μg/mL and 0.3166 μg/mL and 0.2419 μg/mL and 7.98 μg/mL for Hydrochlorothiazide and Fimasartan
respectively. The proposed method is recommended for routine analysis of Hydrochlorothiazide and Fimasartan in
synthetic mixture in regular quality control testing laboratories.

Keywords: Hydrochlorothiazide; Fimasartan; UV; Validation.