European Journal of Biomedical and Pharmaceutical Sciences

MULTIPLE SHOOTS FROM LEAF EXPLANTS OF SOLANUM NIGRUM. L-A WIDELY USED PLANT IN ANTITUMORIGENIC MEDICINE

P. Karnakar Rao, Ramesh Kandagatla, M. Venkat, D. Rajkumar, B. Srinivas, D. Srinivas, *M. Venkateshwarlu

ABSTRACT

MS medium fortified with 2.0 mg/l BAP 0.5 mg/l L-2.0mgl/l Kn or 3.0 mg/l L-G Glutamic acid also induced shoot buds on leaf segments. Sometimes S. nigrum is confused for the more toxic deadly nightshade (Atropa belladonna), which is in a different genus within Solanaceae. A comparison of the fruit shows that the black nightshade berries grow in bunches, whereas the deadly nightshade berries grow individually. Another distinction is black nightshade flowers have white petals. Regeneration shoots from Solanum nigrum .L leaf explants of callus and In Vitro multiple shoots were obtained on MS Medium within BAP, NAA, L-Glumatic acid and Kinetin, Coconut milk (CM). High frequency plant regeneration from leaf explants of S. Nigrum by Ugander, et. al (2010) Callus induction and base explants of Aloe vera R. Prasad, Venkateshwarlu M et.al (2018). MS basal medium supplemented with various Auxons/Cytokinins BAP and NAA. Coconut water also had a rolein triggering the formation of mult1ple shoots. Addition of BAP at 3.0 mg/l and NAA at 2.0 mg/l to the MS basal medium, induced regeneration from the leaf segments. With an increase in the level of BAP 1.0 - 3.0 mg/l the percentage of explants producing shoots also increased. The number of shoots developed on the leaf segments ranged from 1-4 to 2-3 by the addition o BAP at concentration of 1.0 mg/I or NAA at 2.0 mg/l. Among The three concentrations of coconut milk used i.e, 5, 10 and 15% of coconut milk along with 0.5 mg/l BAP proved to be ideal for multiple shoot induction. Cucumber green mottel mosaic virus Wayne, et.al (2011) and Solanum nigrum also suffers from downy and powdery mildews which seriously limits the crop production. Axillary buds from pumpkin were reported by Ugender, et.al (2019) & Rathore (2010). S. nigrum is an important ingredient in traditional Indian medicines. Infusions are used in dysentery stomach complaints and fever. Sikdar, M; Dutta, U (2008) & Read, BE (1977). The fruits are used as a tonic laxative appetite stimulant and for treating asthma and "excessive thirst". Traditionally the plant was used to treat tuberculosis. This plant's leaves are used to treat mouth ulcers that happen during winter periods of Tamil Nadu, India. In North India, the boiled extracts of leaves and berries are also used to alleviate liver-related ailments, including jaundice.

Keywords: Leaf Explants, NAA, L-Glutamic acid, BAP Solanum nigrum L.