RELATIONSHIP BETWEEN REACTIVE OXYGEN SPECIES, VITAMIN E, CATALASE AND CHLAMYDIA TRACHOMATIS IN SERUM OF INFERTILE FEMALE
*Abdul-Wahab Razooqi Hamad, Esraa Abdullatif Alethawi, Hala I. Al-Daghistani, Mayas Khadra
ABSTRACT
Background: The failure in female reproductive system could be cause infertility according to different factors
such as sperm isoantobody, zona pellucid antibody, ovarian antibody, Chlamydia trachomatis infection, hormonal
imbalance and even between oxygen radical and antioxygen radical. These factors might deteriorate female
infertility by different mechanisms. Material and Methods: retrospective case-control study was performed on
105 infertile Jordanian female including 75(71.4%) with primary infertility, and 30(28.6%) with secondary
infertility. Thirty healthy women were used as a control. Enzyme labeled and Biotin labeled antibody kits were
used in the serum and cervical secretion for determination. Polymerase Chain Reaction (PCR) assay was used to
assess the Chlamydia trachomatis in the cervical secretion. Finally, the mean level of Reactive Oxygen Species
(ROS) and anti ROS including Vitamin E, and Catalase (CAT) enzyme were estimated in the cervical secretion
Results: Serum samples obtained from fertile and infertile females were analyzed for the presence of C.
trachomatis specific IgG. 5 (4.76%) of tubal damage infertile females showed the presence of Chlamydial
antibody with no detectable level in the serum of fertile females. To confirm the presence of C. trachomatis, PCR
was used to identify Chlamydia DNA in the cervical secretion. Only positive serum samples revealed clear, sharp
bands of amplified Chlamydial DNA with 241bp. The presence of reactive oxygen species (ROS) within the
cervical secretion cancontribute to female infertility if dose not controlled by anti-oxidants. The results revealed
no statistical difference in the mean level of ROS, and anti ROS among infertility cases and control. However, the
mean antioxidant capacity (vitamin E and catalase) among infertile females with combine infertility problems.
Conclusion: We concluded that isoimmunity to sperm and autoimmunity to ova antigens accompanied by
elevation in the reactive oxygen species play an ambiguous role in fertility impairment.
Keywords: Chlamydia trachomatis, ROS, Catalase, Vitamin E.
[Full Text Article]
[Download Certificate]
Impact Factor : 8.181 
