European Journal of Biomedical and Pharmaceutical Sciences

STUDY OF SOME IMMUNOLOGICAL ASPECTS IN PATIENTS INFECTED BY S.EPIDERMIDIS FORM BIOFILM

Dr. Rajwa Hasen Essa, Dr. Suzan Saadi Hussain and Dr. Nihad Khalawe Tektook*

ABSTRACT

Background: Staphylococcus.epidermidis is one of coagulase negative staphylococci (CONS) are now well established as major nosocomial pathogens associated with infections of indwelling medical devices, In recent years many studies focused on S.epidermidis because of its ability of to form biofilm , which are essential step in the pathogenesits of this bacteria therefore this study aimed Isolated bacteria from different source identificated, so study effect biofilm of this bacteria on non- specific immune response through estimate levels of IL 6 and IL 8 and C- reactive protein, on specific immune response through determine Immunoglobulins (IgG, IgM, IgA and Complement concentration (C3and C4). Results: A total of 50 isolate of staph. Epidermidis isolated from different sources include blood, catheter urine specimen, wound as well as burn swabs and swabs of skin and nasal hospital staff, these sample had been cultured and identified by using VITEK-2 system within 6 h. Blood samples occupied the first place in isolation S.epidermidis 60%, followed by catheter urine in the second place 22%. Most Staphylococcus epidermidis was isolated from male patients (58%) compare to female (42%), prevalence of Staphylococcus epidermidis with age were peaked in the t h i r d g r o u p (4 6 - 65) years, as well as male preponderance in all years age group, excepted in third group, the proportion of females (65%) appeared higher than it is in males (35%). so 60% bacterial isolate were positive biofilm produced by microtiter plate. Normal level both immunoglobulin M and A (90.23±44.23, 124.4±41.20 and 220.8±90.209, 200.10±79.004) mg / dl respectively(p>0.001), but IgG increased level is highly elevated (1535±812.333, 1521.3±475.440) mg / dl respectively(p< 0.001) in sera of patients with produced and non-produced biofilm, and normal levels of (C3) (120±36.5, 120±36.49) mg/dl respectively (P> 0.001), in sera of patients with produced biofilm compare to non-produced biofilm whilst elevated levels of (C4) (41.9±14.09 42±14.28) mg/dl respectively (p>0.001)to each two groups. (produced and non-produced biofilm), so level of cytokines (IL-6, IL-8) appear variations between patients and controls, levels of IL-6 (24.8 ±18.20 , 24.5 ±19.09) pg/ml respectively in sera of infected patients by S.epidermidis (produced and non- produced biofilm) respectively compare to the control (15.88±6.8) pg/ml(P< 0.001), while serum level of IL-8 (38±9.52,37±7.13) pg/ml respectively in sera of infected patients by S.epidermidis (produced and non – produced biofilm) respectively compare to the control (20.3±7.07) pg/ml (P< 0.001). Conclusions: I can conclude from our study that level of immunoglobulin and complement in study group were within the normal range excepted (IgG and C4) so S.epidermidis induced higher levels of IL-8, as well as IL-8 correlated positively with CRP and IL-6 in patients.

Keywords: Staphylococcus.epidermidis, interleukin: IL, C- reactive protein: CRP, Immunoglobulin: I.g,