European Journal of Biomedical and Pharmaceutical Sciences

ORAL ADMINISTRATION OF PETROLEUM ETHER EXTRACT OF TECOMELLA UNDULATA LEAVES AFFECTS SPERMATOGENESIS AND FERTILITY OF MALE RATS.

Pawan Kumar Soni and Pratap Chand Mali*

ABSTRACT

The 50% petroleum ether extract of Tecomella undulata leaves was administered orally at the dose level of 50,100 and 200 mg/kg body wt/day to male rats for 60 days to search a reversible male contraceptive agent. The result reveals that do not caused any significant change in their body weights, however, the weights of reproductive organs i.e. testes, epididymides, seminal vesicles and ventral prostate were decreased significantly as compared to controls. Sperm motility and sperm density in cauda epididymides was significantly declined which resulted in reduction of fertility. Level of testosterone and LH hormones were significantly reduced after the treatment of T.undulata leaves extract. The level of protein, sialic acid, glycogen and cholesterol contents were significantly decreased in testis and other accessory sex organs with the T.undulata leaves extract treatment. There was no significant alteration was observed in SGOT, SGPT, acid phosphatases, alkaline phosphatases, cholesterol and LDH level in the serum of treated rats. Histoarchitecture of the testis exhibit degenerative changes in germinal epithelium and all spermatocytes stage of spermatogenesis support the decreased sperm density in treated rats. Intertubular space was increased in between seminiferous tubules as compared to controls. In conclusion, oral administration of T.undulata leaves extract for 60 days caused androgen deprivation affects spermatogenesis in treated rats without caused any side effects might be due to antiandrogenic nature of the drug.

Keywords: Tecomella undulata, fertility, spermatogenesis, rats, hormones.