European Journal of Biomedical and Pharmaceutical Sciences

THE GENETIC FIDELITY ASSESSMENT OF AILANTHUS EXCELSA’S LEAVES BETWEEN MANDSAUR (M.P.) AND RATLAM (M.P.) DISTRICTS

Bhatt Shashank*, Dhyani Suresh and Kesharwani RK

ABSTRACT

The Ailanthus excelsa Roxb. is found in different states in India and their leaves are used as fodder for cattle. 95% ethanolic extract was shown as a good solvent having a much extraction capacity of secondary metabolites from dried leaves. The glycosides, phenol, lignin, saponins and tannins were present in the 95% ethanolic extract of dried leaves. The concentrations of DNA were found 0.0218, 0.0210, 0.0207, 0.0215 and 0.0212 mg/100gm respectively and their mean value was found 0.0212 mg/100 gm in Mandsaur area of Madhya Pradesh where as in the area of Ratlam, the DNA concentrations were found 0.0218, 0.0212, 0.0218, 0.0218 and 0.0223mg/100gm respectively and the mean value was 0.0218 mg/100 gm. The comparative study between Mandsaur (M.P.) area and Ratlam (M.P.) area were calculated by ANOVA-CRD in which the S.EM. value was 0.006289 and CD 5% 0.018553. These datas showed a significant value.

Keywords: Secondary Metabolites, Genomic DNA, Ailanthus excelsa Roxb.