European Journal of Biomedical and Pharmaceutical Sciences

ANTIMICROBIAL ATTRIBUTES IN VIVO GROWN CATHARANTHUS ROSEUS LINN

Priyanka Tolambiya* and Sujata Mathur

ABSTRACT

Objective:- Identification of antimicrobial attributes in vivo grown Catharanthus roseus in presence of polar and non polar solvent and performance of Catharanthus roseus plant extracts i.e. root, stem, plant, flower against different bacteria's and fungi's micro-organisms. Method:-Antimicrobial activity of C. roseus was studied in presence of polar and non polar solvent i.e. methanol(CH3-OH) and chloroform(CHCl3) extracts against four bacterial i.e. Bacillus subtilis(B. subtilis), Staphylococcus aureus(S. aureus), Escherichia coli(E. coli), Streptomyces griseus(S. griseus) and four fungal i.e. Fusarium oxysporum(F.oxysporum), Trichoderma reesei (T. reesei), Penicillium funiculosum (P.funiculosum), Aspergillus niger(A. Niger) strains were selected for the primary screening. Results:- By measuring the zone of inhibition using agar well diffusion method it have been observed that both polar(methanol) and non polar(chloroform) solvent extracts showed a very strong antimicrobial activity against both bacteria and fungi. The strongest inhibition activity of the methanolic root extract was observed against bacteria's named as S. aureus & E. coli (20 mm inhibition zone each) at IC 400 mg/100ml. Methanol root extract also demonstrates antifungal activity against the pathogenic fungi T.reesei (10 mm zone of inhibition) at IC 400 mg/100ml root extract and in P. funiculosum(12 mm zone of inhibition) at IC 20mg/10ml stem extract. Similarly chloroform leaf extract was observed against the bacterial strain E. coli (20mm zone of inhibition) at IC 230mg/100ml and S. aureus (16 mm zone) at IC 230 mg/100ml of leaf extract. Conclusion:- The Quantitative and Qualitative analysis of C. roseus plant extract screening reveals that there are active component like Flavonoids, Sterols, Alkaloids and Phenolic in C. roseus and it has a enormous potential of antimicrobial attributes in vivo condition that could be inhibit the growth of fungi and bacteria and polar solvent (Methanol) can be used as antimicrobial resource. Also we can conclude that the lower the specific gravity of solvent having higher inhibition possibility with C. rouses extracts.

Keywords: Antimicorbial; inhibition; concentration; Solvent; Bacteria; fungi; Catharanthus roseus Linn; pharmaceutical; Phenolic & Sterol compound; Flavonoids; Alkaloids; micro-organisms.