Abstract
SPECTROPHOTOMETRIC METHODS FOR THE SIMULTANEOUS ESTIMATION OF AMIKACIN AND CEFEPIME IN PHARMACEUTICAL FORMULATIONS

L. Kalyani and Chava V. N. Rao*

ABSTRACT

Simple and sensitive spectrophotometric methods have been developed for the quantitative estimation of Cefepime and Amikacin in pharmaceutical formulations. Method I and II are simultaneous analysis of Cefepime and Amikacin in UV region based on simultaneous equation method and first order derivative spectrophotometry method respectively. III and IV methods are visible spectrophotometric methods for the estimation of Cefepime and Amikacin individually. Simultaneous equation method under Ultraviolet condition was proposed with water as solvent and found to have absorbance maxima at 210 nm for Amikacin and 217 nm for Cefepime respectively. First order spectrophotometric method was applied for simultaneous estimation at 210 nm for Amikacin and 217 nm for Cefepime respectively. The linearity lies between 5-17.5 μg/ml for Amikacin and 20-70 μg/ml for Cefepime in both simultaneous UV and first derivative methods. Visible spectrophotometry method for Amikacin was proposed with p-Dimethyl Amino Benzaldehyde (PDAB) Method at 595 nm and for Cefepime it was proposed with Wool Fast Blue BL (WFBBL) method at 498 nm. The results of analysis have been validated statistically as per ICH guidelines. The accuracy and precision of the methods were determined and validated statistically. All methods were found to be rapid, specific, precise and accurate and can be successfully applied for the routine analysis of Cefepime and Amikacin in pure and combined dosage form.

Keywords: Cefepime, Amikacin, Spectrophotometry, Method development, Validation.


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