European Journal of Biomedical and Pharmaceutical Sciences

DETECTION OF BIOFILM FORMATION AND ITS RELATED WITH AMINOGLYCOSIDE RESISTANCE IN ACINETOBACTER BAUMANNII ISOLATES, ISOLATED FROM SOME BAGHDAD CITY HOSPITALS

Lamieaa G.H. Fajir*, Asmma E. Al-Niaame and Nihia Neama Hussein

ABSTRACT

In the last decade the health care sections has face a new strong threaten by new nosocomial as multi-drug resistant known as Acinetobacter baumannii, this organisms has the ability to reinforce it colonization by the producing of biofilm result in resistant to many classes of the antibiotics. 125 samples has been collected from Baghdadian hospitals from patients with skin infections (burns, bedsores, diabetic foot ulcers, and wounds), it first examine by microscope, and after the biochemical tests and identification by the mechanical VITEK2, an18(14.4%) isolates was known as Acinetobacter baumannii, the ability of the isolates to produce the biofilm has been studied by three methods Congo Red Agar (CRA) Tube Method (TM), and Tissue Culter Plate (TCP), all the isolates were show the ability to produce the biofilm at less in one way, with several degrees ,the sensitivity to biofilm production was in rate0%, 100%, 100%, specifity 0%, when the false positive 0% and the false negative 0%and the positive predictive value was 100% and the negative predictive value was 0% for the three methods, the susceptibility to the Aminoglycoside antibiotics has been study to five antibiotics (Gentamicin GM, Tobramycin TOB, Netlimicin NET ,Amikacin AK ,and Streptomycin S),the isolates show resist to the Gentamicin and Tobramycin in 100 percent , followed by the Streptomycin 77.7%, Amikacin 72%, and the Netlimicin 50%, the correlation between the resistance to the antibiotics and biofilm production has been study. There was a correlation between the ability of production of biofilm and the resistance to the Aminoglycoside antibiotics production but there was no connection between how much the biofilm is strong and the resistant to the aminoglycoside production.

Keywords: Acinetobacter, baumannii, skin infections, Biofilm, Antibiotics resistance, Aminoglycosides, Baghdad hospitals, Vitek 2 compact system.