Abstract
BIOCHEMICAL CHANGES IN LEBISTES RETICULATUS DUE TO CADMIUM STRESS

Sabiha Khan*

ABSTRACT

The fish Lebistes reticulatus was exposed to sub-lethal concentration of 8x10-3 μg/ l cadmium (probit method). Autopsy of liver and kidney was done after 30th days. A decrease in SDH activity levels is due to accumulation of the succinate. Decreased GDH activity in both tissues is an indication of decreased oxidation of glutamate. Reduction in GDH activity may also be due to low utilization of amino acids and low oxidation of amino acids. Decrease in MDH levels in both tissues might be due to restricted supply of substrate, since it was observed that SDH activity decreased. Decrease in MDH may be due to diversion of aerobic metabolism at the level of malic acid, leading to the production of pyruvic acid. The elevation in the activity levels of LDH in both tissues indicates an increased production of pyruvate (or) greater breakdown of lactate. Increase in LDH activity might be due to high energy demand for survival in anaerobic conditions and to overcome cadmium toxicity. The higher level of LDH may be due to elevated rate of enzyme synthesis it is probably reflecting enhanced rate of gluconeogenesis.

Keywords: Cadmium, Sublethal, Autopsy.


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