European Journal of Biomedical and Pharmaceutical Sciences

NUTRITIONAL COMPOSITION, ENZYME ACTIVITY AND ANTIMICROBIAL ACTIVITY OF WOOD APPLE (AEGLEMARMELOS) LEAVES

Jabin Jahan*, Shweta Biswas, Md. Mahabur Rahman, Md. Omar Faruk, Mrityunjoy Biswas and Dr. Farzana Parvin

ABSTRACT

Wood Apple leaves, a medicinal and auyrvedic leaves is locally known as „Bael leaves‟ we investigated nutritional composition, enzyme activities and biological activities (antimicrobial) of locally available Wood Apple (Aeglemarmelos) leaves. The nutritional composition of Wood Apple leaves was determined by standardmethod. On TLC, it was found that Ethyl-acetate (EA) and Methanol(M) extracts of Wood Apple leaves was a mixture of different type of compounds like steroid and terpenoid. Enzyme activity was also determined .The activities of amylase cellulase, invertas and protease were 0.251, 0.132, 0.075, 0.0512 mg/min/ml respectively. In vitro, antibacterial activity of crude methanol extract (ME) was determined against four gram positive and five gram negative bacteria. Leaves extract have antibacterial activity against Shigella dysenteriae, Sarcinalutea, Escherichia coli, Bacillus megaterium, Salmonella typhi. It was found that leaves extract showed no sensitivity againstfive tested fungi.

Keywords: Standard method, TLC, Enzymatic activity, Antibacterial activity, Antifungal activity.