European Journal of Biomedical and Pharmaceutical Sciences

EFFECT OF LAWSONIA INERMIS LEAVE EXTRACT IN ALUMINIUM- INDUCED OXIDATIVE STRESS ON BIOCHEMICAL PARAMETERS OF ADULT MALE WISTAR RAT.

Olawuyi T. S.*, Olorunfemi O. J. and Aina O. S.

ABSTRACT

Henna (Lawsonia inermis) is a perennial shrub, that is mildly anti-inflammatory and is effective against ringworm and other fungal diseases. All isolated compounds exhibited antioxidant activity. Toxic effects of aluminum poisoning can cause asthenospermia, hypospermia, teratospermia and reduction in sperm count. The aim of this study was to investigate: ‘Effect of Lawsonia inermis Leaves Extract on Aluminium-induced Oxidative stress on biochemical parameters of Adult male Wistar rat’. The objectives were to study the antioxidant effect of Lawsonia inermis aqeuous leaves extract on aluminum induced oxidative stress on the Biochemical parameters: Superoxide dismutase (SOD), Glutathione peroxidase (GPx), Malondialdehyde (MDA). Thirty five adult male Wistar rats were used, with an average weight of 100-196g. Lawsonia inermis extracts and aluminum chloride (AlCl3) were administered for a period of three (3) weeks with five (5) rats per group.Group A (control): Given rat pellets and distilled water. Group B: Given 60mg/kg/d extract of aqeuous Lawsonia inermis. Group C: Given0.5mg/kg/d of AlCl3. Group D: Given 0.5mg/kg/d of AlCl3and low dose 60mg/kg/dof aqueous Lawsonia inermis orally. Group E: Given 0.5mg/kg/d of AlCl3and medium dose 75mg/kg/d of aqeuous Lawsonia inermis orally. Group F: Given 0.5mg/kg/d of AlCl3 and high dose 100mg/kg/d of aqueous Lawsonia inermis orally. Group G: Given 0.5mg/k/d of AlCl3and 5mg/Kg/d Ascorbic acid in distilled water orally. Twenty four hours after the last administration, the animal were weighed and thereafter sacrificed by the use of chloroform as a sedative. The organs were located, removed and weighed using an electronic sensitive analytical balance (Sartorious). Results, biochemical findings shows that Superoxide dismutase and Glutathione Reductase were markedly significant (p<0.05). In conclusion, Lawsonia inermis aqueous leaf extract protects the cells and organ systems of the body against reactive oxygen species harmless, a good antioxidant agent against oxidative stress induced by heavy metal toxicity.

Keywords: Asthenospermia, Hypospermia, L. inermis, Peroxidation, Teratospermia.