European Journal of Biomedical and Pharmaceutical Sciences

KISSPEPTIN ACTS AS A COMMON MESSENGER IN THE PATHWAYS CONTROLLING NUTRITION AND REPRODUCTION IN MALE MOUSE

Shabana Anjum and Amitabh Krishna*

ABSTRACT

The aim of the present study was to evaluate the role of Kisspeptin (KP-10) as a modulator of changes in adipose tissue and testicular activity. To achieve this, mice were treated with KP- 10 with different doses (20 pg, 2ng and 2μg/day) for 15 days and evaluated the metabolic and hormonal changes in adipose tissue and testis.The results of this study showed differential effect of KP-10 on changes in metabolic factors of adipose tissue and testis of mice. In adipose tissue, KP-10 promoted uptake of nutrients (triglycerides and glucose) from circulation and resulted in increased accumulation of white adipose tissue. Whereas in the testis, KP-10 caused down-regulation of GLUT8 expression resulting in decreased uptake of glucose, which in turn resulted in decreased synthesis of testosterone. The mice treated with KP-10 showed decreased synthesis of adiponectin in the adipose tissue and decreased expression of AdipoR1 in the testis. The decreased adiponectin synthesis in adipose tissue in turn may be responsible for decreased testicular activity in the mice. The in vitro treatment of KP-10 showed increased uptake of nutrients in adipose tissue but inhibitory changes in testis. These findings thus suggest that KP-10 showed direct role in modulating energy balance in adipose tissue, which is reciprocally linked to testicular activities in mice.

Keywords: Kisspeptin, Adiposity, GLUT8, Adiponectin, testis, white adipose tissue.