Abstract
"DISCERNMENT OF PLASMID-MEDIATED MULTIDRUG RESISTANCE IN ESCHERICHIA COLI ISOLATED FROM HOSPITAL EFFLUENTS"

Amritha G. Kulkarni, Mohith Sen Bhandarkar and A.B. Vedamurthy*

ABSTRACT

Most of the original antibiotics have been rendered obsolete due to antibiotic overuse, abuse, and misuse. Hospital effluents and sewage are an excellent compartment for the selection of Multidrug-resistant(MDR) opportunistic, facultative pathogens such as Escherichia coli, which cause many of the nosocomial infections. The present study reveals the resistance profile of multidrug-resistant Escherichia coli isolated from hospital wastewater effluents of different hospitals in north Karnataka and the factors conferring multidrug-resistance. Several strains of Escherichia coli were isolated from hospital effluents and analyzed by agar disc diffusion to determine their susceptibility patterns to antimicrobial agents commonly used in these hospitals. The strain of Escherichia coli S-2 identified as Escherichia coli-SUS5EC by 16s-rRNA sequencing was found to be resistant to maximum number of antibiotics such as Ceftriaxone, Cefotaxime, Erythromycin, Penicillin, Amoxicillin, Ampicillin, Azithromycin out of thirteen antibiotics used and hence proven to be a multidrug-resistant mutant of Escherichia coli. The Plasmid DNA was isolated and plasmid mediated multidrug-resistance was confirmed by plasmid curing with the above-mentioned antibiotics. This finding substantiates the hypothesis that hospital sewage effluents contribute to the dissemination of transferable multidrug-resistance in bacteria. Thus, public health potential and the possible impact of Multidrug-resistant commensal Escherichia coli and other bacteria occurring in hospital effluents can be a major threat to the treatment of disease by commercially available antibiotics and is a major concern which needs further investigation and monitoring and the mechanisms for such diffusion, and methods to block the entry of such strains into the human population and their subsequent spread, need to be defined.

Keywords: Hospital Effluents, Antibiotic resistance, Multidrug resistant, Escherichia coli, Plasmid, Plasmid Curing.


[Full Text Article]

Login





Forgot Password  |  Register

Indexing

Best Paper Awards

European Journal of Biomedical and Pharmaceutical Sciences (EJBPS) will give best paper award in every issue in the form of money along with certificate to promote research activity of scholar.

Best Article of current issue :

Dr. Dhrubo Jyoti Sen

Download Article : Click here

News & Updation

  • EJBPS: FEBRUARY ISSUE PUBLISHED

    FEBRUARY 2023 Issue has been successfully launched on 1 FEBRUARY 2023.

  • EJBPS New Impact Factor

    Its our Pleasure to Inform you that EJBPS Impact Factor has been increased from 5.467 to 6.044, due to high quality Publication at International Level.

  • Index Copernicus Value

    EJBPS Received Index Copernicus Value 77.3, due to High Quality Publication in EJBPS at International Level

  • Journal web site support Internet Explorer, Google Chrome, Mozilla Firefox, Opera, Saffari for easy download of article without any trouble.

    .

  • Article Invited for Publication

    Dear Researcher, Article Invited for Publication  in EJBPS coming Issue.

     

UG/PG/Ph.D Research Publication

Research Scholar of UG/PG/Ph.D can Submit their Research Article/Review Article/Case Study/Short Communication for Publication in EJBPS

Downloads

Copyright From

Covering Letter

                        Author Instruction 
 

PLAGERLUM REPORT