Abstract
PHYTOCHEMICAL SCREENING, ANTIBACTERIAL ACTIVITY AND GC/MS ANALYSIS OF OIL FROM SUDANESE OKRA

Dr. Tuhami E. Hagr*, I. S. I. Adam, Eltoum E. M. Elnour and Malik S. Hamid

ABSTRACT

This investigation was carried out, for phytochemical screening and to study the chemical constituents of Sudanese Okra Abelmoschusesculentus Oil and to assess its potential antibacterial activity. Preliminary phytochemical screening of ethanolic crude extract from Okra Seeds indicated the presence of Alkaloids, Flavonoids, Carbohydrates, Saponins, Triterpens, and Steroids. Maceration extraction of Abelmoschusesculentus (L) seeds whih is done with n-hexane gave 11ml of Oil. The twenty three components were detected by GC-MS analysis. Major constituents are: 9,12-Octadecadienoic acid(Z,Z)-, methyl ester (31.42%), Hexadecanoic acid, methyl ester (27.56%), 9,-Octadecadienoic acid (Z)-, methyl ester(13.32%), methyl stearate(8.17%), Tricyclo[20.8.0.0(7,16)]triacontane, 1(22), 7(5.98%), Cis10Nonadecenoicacid, methylester(2.62%), 9-Octadecenoic acid, methyl ester, (E)-(1.61%), PGH1, methyl ester (1.60%), Eicosanoic, acid, methyl ester(1.59%), 9,12-Octadecadienoyl chloride, (Z,Z)-(1.42%), Methyl tetradecanoate (0.90%), Docosanoic acid, methyl ester(0.92%), 9-Hexadecenoicacid, methylester, (Z)(0.78%), Cis10Heptadecenoicacid, methylester(0.66%), Tetracosanoic acid, methylester (0.46%), Heptadeconicacid, methylester(0.44%), Methyl 9-12-heptadecadienoate(0.22%), Tricosanoic acid, methyl ester(0.12%), Pentadecanoic acid, methyl ester(0.10%), 7-Hexadecenoic acid, methyl ester, (Z)-(0.06%), 6-Octadecenoic acid methyl ester(0.03%0, 4-Octadecenoic acid, methyl ester (0.02%), Dodecanoic acid methyl ester (0.01%). The oil Abelmoschusesculentus (L) seeds showed activity against four human pathogens organisms. Significant activity was observed against the Gram positive bacteria: Bacillus subtitles'.

Keywords: Phytochemical screening, Antimicrobial activity, Abelmoschusesculentus, GC/MS analysis.


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