European Journal of Biomedical and Pharmaceutical Sciences

EVALUATION OF ANTIBACTERIAL AND ANTIOXIDANT ACTIVITIES OF PARTIALLY PURIFIED LECTIN FROM TUBERS OF XANTHOSOMA VIOLACEUM

Anitha N. and Sathisha G. J.*

ABSTRACT

The present work describes the antimicrobial and antioxidant activities of a partially purified lectin from tubers of Xanthosoma violaceum (XVL). XVL was partially purified by ammonium sulphate precipitation followed by dialysis. Partially purified lectin was assayed for antibacterial activity against bacteria Escherichia coli, Salmonella typhimurium, Bacillus subtilis and Pseudomonas aeruginosa. The antibacterial activity was studied using agar well diffusion method. Further, antioxidant activity was performed by 2, 2-diphenyl-1-picrylhydrazyl (DPPH) method, iron chelating and superoxide radical scavenging activity. XVL exhibit antibacterial activity against Escherichia coli, Salmonella typhimurium and Bacillus subtilis but not towards Pseudomonas aeruginosa. The results of antioxidant activity of XVL were expressed in terms of percentage of inhibition. Therefore, XVL found to be a promising antibacterial and antioxidant agent which can be used as a natural medicinal product.

Keywords: Xanthosoma violaceum, antimicrobial, antioxidant, lectins, tuber.