Abstract
DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR ESTIMATION OF PERINDOPRIL ERBUMINE IN PHARMACEUTICAL DOSAGE FORM

Dandamudi Santhoshi Priya*, Suchitra D., Sahithi A., Parijatha B., Himaja M. and Krishna Mohan Chinnala

ABSTRACT

A new method was established for estimation of Perindopril erbumine by RP-HPLC method. The chromatographic conditions were successfully developed for the separation of perindopril by using Xterra C18 4.6×250 mm 5.0 μm, flow rate was 0.8ml/min, and mobile phase ratio was 65:35%v/v methanol: water, detection wavelength was 218nm. The instrument used was SHIMADZU HPLC, software N-2000.The retention times were found to be 2.482 mins. The analytical method was validated according to ICH guidelines [ICH, Q2 (R1)]. The developed method was found to be simple, rapid, accurate, linear, and précised. The linearity study of perindopril was found in concentration range of 30μg-150μg and correlation coefficient (r2) was found to be 0.997, % recovery was found to be 100.3%. LOD value was 2.97μg/ml and LOQ value was 9.92μg/ml. Hence the suggested RP-HPLC method can be used for routine analysis of perindopril in standard and pharmaceutical dosage form.

Keywords: Perindopril erbumine, RP-HPLC, Xterra C18 and retention time.


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