Abstract
METAL REMOVAL AND ANTIMICROBIAL EFFICACY OF RHAMNOLIPID PRODUCED BY CITROBACTER SEDLAKII D5 USING AGRO-INDUSTRIAL WASTES

Subramaniyan Murugajothi and Selvaraj Radhathirumalaiarasu*

ABSTRACT

Biosurfactants were selected as an attractive tool because of its versatility, biodegradability, ecological safety and environmental acceptance. Since analyzing its ability to use agro-industrial wastes is needed for its mass production and industrial application, this work aimed to optimize the production of Biosurfactant with different agro wastes. The potent isolate D5 was identified based on 16S rRNA gene analysis and confirmed with blast that it closely related to C. sedlakii (99%). Among the agro waste tested, C. sedlakii D5 exhibited maximum biosurfactant production utilizing sugarcane bagasse and peanut waste with the highest zone formation in oil spreading technique and emulsification activity (25 mm and 78.5%). Further the biosurfactant of 160 mg/200 ml was extracted and the Biosurfactant was characterized as rhamnolipid with phenol-sulphuric test and TLC analysis. The amount of metal removed after biosurfactant treatment were found to be 15-45% for manganese, copper, zinc, silver and mercury. The Biosurfactant of C. sedlakii elaborated remarkable antibacterial activity with zone of inhibition of 23mm and 16 mm against E. coli and Staph. aureus. The outcome of this work emphasis the use of sugarcane bagasse and peanut as cost effective and sustainable agro-industrial substrates for biosurfactant production and suitability of C. sedlakii D5 as ideal candidate proven to be further explored for pharmaceutical and environmental application.

Keywords: Rhamnolipid, C. sedlakii, metal removal, antibacterial activity, environmental application.


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