European Journal of Biomedical and Pharmaceutical Sciences

EVALUATION OF ANTIOXIDANT, ANTIBACTERIAL, ANTI- INFLAMMATORY AND ANTI-ARTHRITIC ACTIVITY OF JUSTICIA TRANQUEBARIENSIS

D. Leema Rose Mary*, P. Marie Arockianathan, Priya Nagappan, V.Vinodini, R. Kanaga

ABSTRACT

Plants give many important therapeutic products to improve our health system without any adverse effects. In the
present study the aqueous, methanol and ethanol extracts of the Justicia tranquebariensis was prepared and tested
for various phytochemical and biochemical analysis. The phytochemical analysis revealed the presence of
Saponins, alkaloid, flavinoids, Phytosterols, gum and mucilage in all the extracts of plant. In biochemical analysis
the anti-oxidant assay by DPPH scavenging method, anti-microbial activity by disc diffusion method, Invitro antiinflammatory
activity by HRBC stabilization method and in-vitro anti-arthritic activity by bovine serum albumin
(BSA) denaturation method were analyzed. The results showed the methanolic and ethanolic extracts of plant
exhibit maximum activity in all the parameters with respect to aqueous extract. The methanol extracts of Justicia
Tranquebariensis was tested for anti-bacterial activity against control Ciprofloxacin shows maximum zone of
inhibition. From these results, it was found methanolic and ethanolic extracts of plant shows good anti-oxidant,
anti-inflammatory and anti-arthritic properties. So this plant extract can be exploding further to produce herbal
active principles for therapeutic use.

Keywords: Antioxidant activity, antibacterial activity, anti- inflammatory activity, anti-arthritic activity, Justicia tranquebariensis.