European Journal of Biomedical and Pharmaceutical Sciences

THIN LAYER CHROMATOGRAPHIC PROFILES AND ANTIOXIDANT CAPACITY OF LEAF EXTRACTS OF NELUMBO NUCIFERA GAERTN. (NELUMBONACEAE) FROM CÔTE D’IVOIRE

Modeste Bosson Tano*, Guy Roger Mida Kabran, Marcel Koffi Konan, Apollinaire Koffi Yeboue, Janat Akhanovna Mamyrbekova-Bekro* and Yves-Alain Bekro

ABSTRACT

Within the framework of therapeutic plants valorization from Côte d'Ivoire, Nelumbo nucifera used in traditional medicine to handle diverse pathologies was studied. Phytochemical screening using TLC revealed the co-presence of, alkaloids, coumarins, flavonoids, saponins, sterols, tannins, terpenes and phenolic acids in the selective leaf extracts from N. nucifera. Total phytophenol contents (12992 μg GAE / g), flavonoids (4.494%), anthocyanins (0.865 mg / g), flavonic aglycones (1.87 mg / g) and total tannins (8.721%) have been determined by spectrophotometric method. The antioxidant capacity evaluated with respect to the DPPH, showed that ethyl acetate (RC50 = 0.012 μg / ml) and n-butanol extracts (RC50 = 0.0125 μg / ml) are significantly more active than the hexane (RC50 = 0.11 μg / ml) and chloroform (RC50 = 0.05 μg / ml) extracts. However, the RC50 of ethyl acetate and n-butanol extracts showed no significant difference with regard to vitamin C (RC50 = 0.0117 μg / ml), the positive control.

Keywords: Nelumbo nucifera, leaf extracts, phytochemistry, antioxidant activity, DPPH.