EVALUATION OF IN VITRO ANTIOXIDANT AND ANTI-UROLITHIATIC POTENTIAL OF VARIOUS FRACTIONS OF CLITORIA TERNATEA L. BLUE FLOWERED LEAVES
Priyanga R., Hansiya V. S., Uma Maheswari P., Suman T. and Geetha N.*
ABSTRACT
The present study comprises preparation of hot crude plant extract from Clitoria ternatea L. blue flowered leaves and successive fractionation of the filtered extract using various solvents. All the fractions used for preliminary phytochemical tests as well as for quantification some important secondary metabolites. In addition, in vitro antioxidant and anti-urolithiatic potentials of all the fractions were evaluated. During qualitative phytochemical analysis, all the fractions displayed all the phytocompounds tested such as alkaloids, flavonoids, phenols, tannins, sterols, saponins and terpenoids. All the fractions contained an adequate level of quantitatively determined secondary metabolites such as total phenols, flavonoids, terpenoids and tannins. All the fractions showed varied level of antioxidant activities assayed. Interestingly, among various fractions, n-butanol fraction only exhibited significant inhibition of nucleation, aggregation and growth of CaOx crystals. This may be due to significant free radical scavenging activities of this fraction for total antioxidant capacity, ABTS•+, nitric oxide and DPPH which would further strengthen its use to ameliorate urolithiasis induced oxidative stress.
Keywords: Clitoria ternatea, Blue flowered variety, Phytochemical analysis, In vitro antioxidant activity, Anti-urolithiatic potential.
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