COMPARATIVE EVALUATION OF POULTRY MANURE AND NPK FERTILIZER ON HYDROGEN CYANIDE CONCENTRATION, TOTAL PROTEIN AND RHODANESE ACTIVITY IN TOMATO (SOLANUM LYCOPERSICUM L.) LEAVES
Mohammed Adewumi Abdulrasak, Adeola Folashade Ehigie* and Ona Leonard Ehigie*
ABSTRACT
Green house experiments were conducted in the Department of Agronomy, Ladoke Akintola University of Technology on Tomato (Solanum lycopersicum L.) to evaluate the effect of two selected fertilizers (Azatobactor and Azospirillum) on total protein, Rhodanese activity and Hydrogen Cyanide concentration. Rhaodanese activity was assayed following the method of Agboola and Okonji (2004) while Hydrogen cyanide and protein concentrations were determined by alkaline picrate colourimeter Bradford methods respectively. The result of this study reveals the response of tomato plant to inorganic fertilizer and manure application. The application of the poultry manure and NPK fertilizers significantly (p<0.05) increased total protein, rhodanese activity while no effect was observed on Hydrogen cyanide concentration in tomato leaves. The mineral fertilization increased total protein concentration from 445.15 ± 23.04mg to 664.37mg ± 35.55mg and rhodanese activity from 0.74U/mg ± 0.03 to 1.75 ± 0.12 while the manure increased the protein concentration to 653.49U/mg ± 20.76 and rhodanese activity to 1.93U/mg ±0.03. Since there was no correlation between rhodanese activity and cyanide content, the presence of rhodanese activity may suggest other functions other than cyanide detoxification.
Keywords: Rhodanese, tomato, cellular respiration and fertilizer.
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