Abstract
COMPARISON OF THE NON-SPECIFIC IMMUNE RESPONSE IN THE HEAD KIDNEY OF EDIBLE CARP AGAINST BACTERIAL INFECTION

Satyalatha B.D.J.* and Viveka Vardhani V.

ABSTRACT

Introduction: Occurrence of diseases in edible carps is due to several factors like decreased resistance, poor water quality and bad managerial methods. The most significant factor effecting the health of fish is the incidence of bacterial pathologies in various organs, mainly the primary lymphoid organ, head kidney. Aeromonas liquefaciens (Bacterium) may behave as opportunistic pathogen by assailing already compromised or streseed paths. Objectives: With the above background, the present investigations were planned to assay protein and DNA profile and histological alterations in head kidney of Labeo rohita. Material and Methods: Four groups of experimental fish were infected with single doses (group A, 10-2 CFW/fish; group B, 10-4 CFU/fish; group C, 10-5 CFU/fish; group D, 10-6 CFU/fish) and two experimental group of fish were infected with multiple doses (4 days interval) (group E, 10-2+10-2 CFU/fish; group F, 10-3 + 10+3 CFU/fish) of A. liquefaciens along with uninfected controls (groups, 66 fish). Six fish from a, b, c, d, e, f groups A, B, C, D and E, F and controls were necropsied on each day of eleven observations. Protein and DNA and histology were studied using standard methods. Results and conclusion: Protein and DNA level in head kidney of experimental fish (groups A, B, C and D) showed a significant increase and decrease in when compared with controls from hour 1 to 216 of infection. Protein level in head kidney showed a significant rise in experimental groups A and B when compared with controls; and in between the experimental groups A and B. Protein level in head kidney showed a significant rise in experimental groups E and F when compared with controls; and in between the experimental groups E and F. The DNA level in head kidney showed a significant decrease in experimental groups E and F when compared with controls and in between the experimental groups E and F. The statistical analysis showed a significant difference in the level of head kidney protein and DNA when comparison was made between singly infected groups B (10-4 CFU/fish) and D (10-6 CFU/fish) and repeatedly infected groups E (10-2 + 10-2 CFU/fish) and F (10-3 + 10-3 CFU/fish).The DNA level in head kidney showed a significant decrease in experimental groups A and B when compared with controls and in between the experimental groups A and B. The statistical analysis showed a significant difference in the level of head kidney protein and DNA when comparison was made between singly infected groups B (10-4 CFU/fish) and D (10-6 CFU/fish) and repeatedly infected groups E (10-2 + 10-2 CFU/fish) and F (10-3 + 10-3 CFU/fish).Infected carps showed lesions and necrosis in head kidney during experimentation. Present investigations indicate a high non-specific immune response in experimentally infected fish compared to controls.

Keywords: Immune response, protein, DNA head kidney, histology, edible carp.


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