Abstract
METHOD DEVELOPMENT AND VALIDATION FOR ESTIMATION OF LAMIVUDINE AND ABACAVIR IN BULK SAMPLES AS WELL AS IN TABLET DOSAGE FORMS BY USING RP-HPLC

K. Sravanthi* and Dr. J. Sangeetha

ABSTRACT

In the study, Lamivudine and Abacavir in tablets and bulk were analyzed using reverse phase high performance liquid chromatography. Different trails were performed with different mobile phases and different columns, peaks were observed and optimized based on the retention time, the optimized conditions were: stationary phase - Dionex C18 column, mobile phase - Methanol (40% volume) and potassium dihydrogen phosphate (60% volume) with 0.1 M strength and 3.5 pH, flow rate – 1 ml per min and detecting wavelength 232 nm. The linearity range was 150 to 450 μg/ml (abacavir) and 75 to 225 μg/ml (lamivudine). The developed technique was applied to tablets. The retention time for Abacavir and Lamivudine were found to be 2.397 min and 3.296 min respectively, accuracy, sensitivity and precision were found significant. During stability studies, extra peaks were well segregated for Lamivudine and Abacavir and hence this method can be applied to study degradation of Lamivudine and Abacavir during quality control analysis.

Keywords: Abacavir, Lamivudine, HPLC, LOQ.


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