EFFECTS OF PERSEA AMAERICAN LEAVES EXTRACTS ON ANTIOXIDANT ACTIVITIES AND DISEASES RESISTANCE OF PSEUDOMONAS AERUGINOSA INFECTED CLARIAS GARIEPINUS
Ukwe I. O. K.* and Irondi P.
ABSTRACT
Disease resistance and antioxidant activities in Pseudomonas infected clarias gariepinus exposed to aqueous extracts of Persea amaericana leaves were assessed. One hundred and twenty (120) C. gariepinus of mean weight 120±13g were infected with 1.0ml of 4.1x104cfu, using 2ml injection syringe. After diseases presence/sign, the infected fish were exposed to four different levels of aqueous P. americana leave extracts (0.0ml, 1.0ml, 1.5ml and 2.0ml) in triplicate, for a period of seven (7) days. Blood samples were collected from the experimental fish via kidney puncture before infection, after diseases presence and day 2,5 and 7 of exposure to P. americana leaves extracts and taken to the laboratory and the following antioxidants were accessed: Catalase (CAT), Glutathione (GSH), Glutathione-S-Transferase (GST), superoxide dismutase (SOD) and Melondialdehyde (MDA) to determine the level of stress imposed on the fish by the P. aeruginosa. All the assessed antioxidants were higher in the fish after the infection with P. aerugionsa and the therapeutic effect of the P. americana aqueous leaves extract on the infected C. gariepinus. At the end of the 7 days exposure (treatment) with the extracts, the antioxidant activities were lower in the treated groups (1.0ml – 2.0ml) compared to the untreated group (0.0ml), and the disease resistance ability were: 0.00 ± 0.00, 100.00±0.01, 91.67±9.71 and 66.67±7.51 in 0.0ml, 1.0ml, 1.5ml and 2.0ml aqueous P. americana leaves extract respectively.
Keywords: Aquaculture, Clarias gariepinus, antioxidants, Disease resistance, P. aeruginosa.
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