European Journal of Biomedical and Pharmaceutical Sciences

EXTRACTIVE SPECTROPHOTOMETRIC DETERMINATION OF COPPER (II) USING 2-(5- BROMO-2- OXOINDOLIN-3-YLIDENE) HYDRAZINE CARBOTHIOAMIDE AS AN ANALYTICAL REAGENT

Parinita Umesh Madan and Vasant Dnyandeo Barhate*

ABSTRACT

A simple, rapid and sensitive spectrophotometric method has been developed for the determination of Cu(II) by using 2-(5-Bromo -2-Oxoindolin-3-ylidene)Hydrazine Carbothioamide as an analytical reagent.2-(5-Bromo-2-Oxoindolin-3-ylidene)Hydrazine Carbothioamide has been synthesized and characterized by elemental and spectral analysis. 2-(5-Bromo -2-Oxoindolin-3-ylidene)Hydrazine Carbothioamide extracts Cu (II) quantitatively (99.92%) into n-amyl alcohol from an aqueous solution of pH range 4.0-6.0 and in the presence of 3cm3 of phosphate buffer (pH 5.0). The n-amyl alcohol extract shows an intense peak at 510 nm (λ max). Beer’s law is obeyed over the Cu(II) concentration range of 1.0-8.0 μg/cm3 The Sandell’s sensitivity and molar absorptivity for Cu-2-( 5-Bromo -2-Oxoindolin-3-ylidene)Hydrazine Carbothioamide system is 25.0ngcm-2 and 2538 L mole-1cm-1 respectively. The composition of extracted species is found to be 1:2 [Cu: 2-(5-Bromo -2-Oxoindolin-3-ylidene)Hydrazine Carbothioamide ] by Job’s Continuous Variation and Mole Ratio Method. Interference by various ions has been studied. The proposed method is rapid, sensitive, reproducible and has been successfully applied for determination of Cu(II) in alloy and pharmaceutical samples.

Keywords: Extractive Spectrophotometry, Cu(II), [2-(5-Bromo -2-Oxoindolin-3-ylidene) Hydrazine Carbothioamide or 5-Bromo Isatin thiosemicarbazone [HBITSC], Samples.