European Journal of Biomedical and Pharmaceutical Sciences

STUDY THE COAGULASE GENE IN STAPHYLOCOCCUS AUREUS ISOLATED FROM DIFFERENT SOURCES BY USING PCR AMPLIFICATION

Aemen Ali Al –Shammary, Marrib Nazih Rasheed, Osamah Mohammed Hasan, Mohammed Ibraheem Nader*

ABSTRACT

Coagulase enzyme is one of the virulence factors of Staphylococcus aureus . This study is based on the molecular analysis of the coagulase encoding gene(coa). The polymorphism of the coa gene existing in different S.aureus have been analyzed in this study. Twenty-four isolates (24) of S. aureus were obtained from ninety-five samples (95) which were collected three hospitals in Baghdad and the General Health Lab. These isolates were collected from different sources (patients, food and drinking water) .They were grouped according to their sources. The clinical samples included, wound swab, burn swab, ear swab, urine and blood. The food samples included poultry meat and cheese.DNA from each sample was isolated and the coa gene was amplified with appropriate primers. The PCR results of 24 coagulase positive S.aureus gave three different size of amplicons variation among different S.aureus isolates of samples, which may be considered as an important criterion while treating Staphylococcal infections or source.

Keywords: Staphylococcus aureus, coagulase gene, polymorphism.