Abstract
GENETIC STUDY OF THE METHYLENETETRAHYDROFOLATE REDUCTASE POLYMORPHISMS IN IRAQI COLORECTAL PATIENTS

Thooalnoon Younes Aljanabi*

ABSTRACT

Forty blood samples of CRC patients were collected, besides blood samples were taken from twenty apparently healthy persons and considered as control groups. Polymerase chain reaction restriction fragment length polymorphism (RFLP-PCR) technique was used to detect MTHFR C677T and A1298C polymorphisms, ELISA kit for homocysteine measurement was used. Genotype analysis of MTHFR A12987C revealed AA: 42.5%, AC: 30% and CC 27.5% among CRC cases and 25%, 40% and 35% among controls, while MTHFR C677T undetectable. Homocysteine measurement revealed significant increase (p<0.05) with CRC risk, 21.351±1.84 nmol/ml among patients and 6.97± 9.16 nmol/ml among controls. In conclusion homocysteine increase significantly among CRC patients. MTHFR 1298 A/C and C/C genotypes have no effect on CRC Iraqi patients, and this probably due to ethnic diversity in this study.

Keywords: Genetic polymorphism - colorectal cancer - methylenetetrahydrofolate - genomic DNA, Iraq.


[Full Text Article]

Login





Forgot Password  |  Register

Indexing

Best Paper Awards

European Journal of Biomedical and Pharmaceutical Sciences (EJBPS) will give best paper award in every issue in the form of money along with certificate to promote research activity of scholar.

Best Article of current issue :

Dr. Dhrubo Jyoti Sen

Download Article : Click here

News & Updation

  • EJBPS: MAY ISSUE PUBLISHED

    MAY 2022 Issue has been successfully launched on 1 May 2022.

  • EJBPS New Impact Factor

    Its our Pleasure to Inform you that EJBPS Impact Factor has been increased from 5.467 to 6.044, due to high quality Publication at International Level.

  • Index Copernicus Value

    EJBPS Received Index Copernicus Value 77.3, due to High Quality Publication in EJBPS at International Level

  • Journal web site support Internet Explorer, Google Chrome, Mozilla Firefox, Opera, Saffari for easy download of article without any trouble.

    .

  • Article Invited for Publication

    Dear Researcher, Article Invited for Publication  in EJBPS coming Issue.

     

UG/PG/Ph.D Research Publication

Research Scholar of UG/PG/Ph.D can Submit their Research Article/Review Article/Case Study/Short Communication for Publication in EJBPS

Downloads

Copyright From

Covering Letter

                        Author Instruction 
 

PLAGERLUM REPORT