IDENTIFICATION OF ACTIVE COMPOUNDS FROM BACILLUS AMYLOLIQUEFACIENS JUSC-02 AGAINST BIOFILM FORMING ORAL PATHOGENS
Tanishka Agrawal*, Priyanka Pandey and Wasim Raja
ABSTRACT
The prevalence of most oral infections arises due to the rising antibiotic resistance of oral bacteria against different kinds of antibiotics, which can be attributed to their biofilm forming ability. The most prevalent illnesses of the oral cavity, dental caries and periodontal disease, are caused by dental plaque. Numerous oral isolates such as Kocuria spp. (JUFC 01), Bacillus sp. (JUFC 10), and Neisseria sp. (JUFC 11) were isolated from Periodontitis, dental plaque, and dental caries individuals. These isolates displayed evident resistance to antibiotics such Trimethoprim, Vancomycin, Clindamycin, and Levofloxacin. The production of biofilm was one of these organisms' distinctive traits. As a result, these isolates' defense mechanism of biofilm formation can be linked to their antibiotic resistance. Secondary metabolites of various LABs were extracted to test their effectiveness against oral isolates because LABs are well-known for their wide range of anti-microbial, antibiofilm, and anti-adhesive characteristics. From pomegranate juice, Bacillus amyloliquefaciens (JUSC-02) was identified. To test for their inhibitory efficacy against biofilm generated by Kocuria spp, Bacillus spp, and Neisseria spp, the crude extract of this B. amyloliqu0efaciens was extracted. To investigate the inhibitory activity of crude extracts of LABs against the oral isolates and the biofilm formed by the isolates, a variety of procedures including Microtitre plate assay, MIC analysis, and antibiotic susceptibility tests were used. The fractions of CEs of LABs were to be separated using TLC method. Fluorescence microscopy and the TLC bioautography assay were used to confirm the separated fractions' inhibitory effects on the oral isolates. The bioactive fractions have been identified by FTIR and LC-MS analysis, indicating that the substance may be a phenolic component.
Keywords: Oral isolates, Lactic acid bacteria, Antibiotic resistance, Anti-biofilm activity, bioactive compounds.
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