Abstract
IN- VITRO OPTIMIZATION OF LOCAL MAIZE CULTIVAR OF MUZFFARABAD, AJK.

Safoora Ishfaq, Syed Dilnawaz Ahmed, Asad Hussain Shah, Rizwan Taj Khan, Syeda Maria Fiaz Bukhari, Isma Hameed, Hira Mubeen, Nazish Awan, Syed Rizwan Abbas and Shahid Raza*

ABSTRACT

This work was carried out to optimize different conditions for surface sterilization of maize (Zea mays L.) explants (seeds) of local variety against microbial contamination. Colorex was used as disinfectant to avoid contamination. Seeds were washed thoroughly with colorex (commercial bleach) with different concentrations like 30%, 50%, 70% and 100% for 30 minutes and then rinsed with distilled and autoclaved water for 20 minutes for three times. Concentration of colorex was the key factor in the sterilization process. The best growth rate was observed in the seeds washed with 30% colorex. Seeds with 100% colorex showed reduced growth.

Keywords: Maize; microbial contamination; surface sterilization; colorex; concentration.


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