European Journal of Biomedical and Pharmaceutical Sciences

COMPARATIVE ANTIBACTERIAL AND ANTIFUNGAL EFFICACY OF 15% ALOE BARBADENSIS MILLER, 0.2% CHLORHEXIDINE (CHX), 18% ETIDRONATE AND 5% SODIUM HYPOCHLORITE AGAINST ENTEROCOCCUS FAECALIS AND CANDIDA ALBICANS

Dr. Dakshita Joy Sinha, *Dr. Natasha Jaiswal, Dr. Kanwardeep Singh, Dr. Agrima Vasudeva, Dr. Jagvir Singh and Dr. Shraddha Gupta

ABSTRACT

The ultimate goal of endodontic treatment is to either prevent the development of apical periodontitis or to create adequate conditions for periapical healing in case the disease is already present. As there is no study to our knowledge comparing the antimicrobial efficacy of 15% Aloe barbadensis miller, 0.2% chlorhexidine (CHX), 18% etidronateand 5%sodium hypochlorite, the purpose of this in vitro study was to evaluate the antimicrobial efficacy of herbal irrigants against Enterococcus faecalis and Candida albicans. The testing of the antibacterial and antifungal efficacy of selected medicaments against Enterococcus faecalis and Candida albicanswas done by the agar disc-diffusion method. Pre sterilized Whatman paper disc, 6mm in diameter were soaked with the test solution and placed onto the previously seeded agar petri plates. Each plate was incubated at 37C for 48 hours. Zones of inhibition were recorded for each plate and the results were statistically analyzed. Saline was used as negative control in this study. Chlorhexidine exhibited maximum antibacterial efficacy followed by sodium hypochlorite and Aloe barbadensis miller. Etidronate showed some amount of antibacterial activity.In case of Candida albicans,Aloe barbadensis millerexhibited maximum antifungal efficacy followed by sodium hypochlorite and Chlorhexidine. Etidronate exhibited minimal efficacy while dimethyl sulphoxide and saline showed no antifungal efficacy.

Keywords: Candida albicans, Enterococcus faecalis, Root canal irrigants.