Abstract
SCREENING AND ASSESSMENT OF LACCASE PRODUCING BACTERIA FROM DIFFERENT ENVIRONMENTAL SAMPLES

Randhir Singh* and Anil Kumar

ABSTRACT

Laccase is a copper-containing polyphenol oxidase that acts on a wide range of substrates. This enzyme is found in
many plant species and is widely distributed in fungi including wood-rotting fungi where it is often associated
with lignin peroxidase, manganese dependent peroxidase, or both. Because of its importance in bioremediation.
These include abundant applications in effluent detoxification, enzymatic conversion of chemical intermediates,
wine clarification degradation of textile dyes etc. In the present study, two potential microbes were isolated on
solid medium containing guaiacol and ABTS for laccase activity out of 10 microbes. Two cultures PHP7 and
PKD5 were selected for molecular characterization was carried out using 16S rRNA gene technology of PHP7
revealed as Bacillus cereus (KU878970.1). Using laccase from PHP7 and PKD5 isolates, explored at industrial
level for decolorization of coloured effluents that significance in environmentally friendly and play critical role as
bioremediation at commercial scale.

Keywords: .


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