European Journal of Biomedical and Pharmaceutical Sciences

COMBINED EFFECT OF KUNGILIUM AND AZADIRACTA INDICA LEAF AND ITS BIOLOGICAL PERCEPTIONS, LARVICIDAL ACTIVITY AGAINST THE CULEX QUINQUEFASCIATUS MOSQUITOES

S. Sujatha*, Seema A., Jenisha J. S., Jenisha R. V., Jebishha P. J. and Mary Nivetha B.

ABSTRACT

Mosquitoes transmit serious human diseases like malaria, filariasis, Japanese encephalitis, dengue haemorrhagic fever and yellow fever causing millions of deaths every year. Extensive use of chemical insecticides for control of vector borne diseases has created problems related to physiological resistance to vectors, adverse environmental effects, high operational cost and community acceptance. The present study was aimed to determine the biological perspectives and larvicidal potential of the combined effect of Azadiracta indica (L.) leaf three solvents extracts with powdered substance of Kungiliyum activity against C. quinquefasciatus mosquioto of fourth and fifth instar larvae in laboratory condition. The rate of larval mortality was maximum noticed on ethyl acetate extract combined with kungiliyium powder treated mosquito larvae. Therefore the current research planned to aim the following objectives such as analyse the preliminary phytochemicals, antibacterial activity, examineed the green synthesis of silver nanoparticles Qualitative estimation of protein from the whole-body mass of treated dead mosquito larvae by SDS-PAGE and elucidation of bioactive compounds from better potential extract of leaf from A. indica by GCMS technique. Investigated the moratlity rate of C. quinquefasciatus mosquito larvae treated with combination of kungilium with three various solvent extracts of A. indica leaf. From the present result showed that maximum zone of inhibition against Bacillus sp., and S. marcesence noticed in ethyl acetate and methanol extract respectively. While the treated dead larvae were subjected in to SDS-PAGE analysis it was clearly showed that total number of protein bands (polypeptides) are maximum eleven bands were noticed in control larvae but other experimental treated larvae possessed minimum number of lowest molecular weighed protein band. Moreover, when treated with ethyl acetate and ethanol subjected dead larvae shows the 34kDa protein bands are thickened intensity was more than other bands. Furthermore, GCMS chromatograph showed fourteen different peak areas at retention time 5.52 to 35.56 minutes. The current study was clearly showed the fourteen different bioactive compounds were elucidated from the ethyl acetate leaf extract, amoung those compounds peak compound such as 3-Heptanol, 3,5-dimethyl- Hence, A. indica leaf ethyl acetate extract formulations are relative less toxic, eco-friendly and mosquitoes are unable to develop resistance and may be used as an alternative to other pesticides for control of vector- borne diseases particularly this extract is act as a mosquitocidal mediator.

Keywords: C. quinquefasciatus, SDS-PAGE, antibacterial activity, GCMS.