EVALUATION OF PREBIOTIC POTENTIAL OF HIBISCUS ROSA SINESIS LEAF MUCILAGE
Atul Payasi, Kanika Dhote*, Vinod Kumar Dhote, Surendra Kumar Jain
ABSTRACT
In the present investigation, it was attempted to establish the prebiotic potential of Hibiscus mucilage and also perform the proximate analysis of the extracted mucilage. Hibiscus mucilage was extracted from the leaves in yield of 3.7% by weight of dry leaf powder and subjected to phytochemical screening and estimation of protein, fat, carbohydrates, ash and moisture using the method reported by AOAC. The mucilage was also evaluated for its DPPH radical scavenging potential and its resistance to hydrolysis by gastric juice (acidic hydrolysis) or by α-amylase (enzymatic hydrolysis). The prebiotic potential of the mucilage was studied by assessing its effect on the growth of various lactobacillus strains. The mucilage was found to contain 17.27% moisture, 5.13% ash, and 76.9% carbohydrates. Proteins and fats were totally absent in the mucilage. The total sugar in the Hibiscus mucilage sample was determined by Fehling’s reagent method and was found to be 69 μg. The IC50 value of the mucilage in inhibiting DPPH radical was found to be 57.01μg/mL. Artificial gastric juice (pH 1) was used to hydrolyze the Hibiscus mucilage as well as inulin. The acidic hydrolysis of inulin was found to be 7.14% while that of the Hibiscus mucilage was found to be 13.89%. The enzymatic (-amylase) hydrolysis of inulin was found to be 12.34% while that of the extracted Hibiscus mucilage was found to be 16.14%. The prebiotic promoted the growth of Lactobacillus strains in varying degree. The highest growth was obtained for L. rhamnosus (2.05 ± 0.02) followed by L. acidophilus (1.99 ± 0.03 Log10CFU/mL) and the least for L. fermentum (1.81 ± 0.03 Log10CFU/mL).
Keywords: Mucilage, prebiotic, lactobacillus, amylase, hydrolysis.
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