European Journal of Biomedical and Pharmaceutical Sciences

MITIGATING OF L-NAME INDUCED CARDIOTOXICITY INWISTAR RATS BY FERMENTED UNRIPE PLANTAIN (MUSA PARADISIACA) JUICE

Arhoghro Ejovwoke Marcellinus*, Berezi E. Peter, Agberia Steve Obruche, Owotgwun Kasirotu Levi, Madock Obebinaru Joshua and Vincent Dipamo Tony

ABSTRACT

L-NAME, also known as Nω-nitro-L-arginine methyl ester, is a well-established inhibitor of nitric oxide synthase. It is commonly employed in animal models to induce experimental cardiotoxicity. The present study investigates the potential protective effects of fermented unripe plantain juice (FUPJ) against L-NAME-induced cardiotoxicity in male Wistar rats. Twenty (20) healthy wister rats weighing within 110g to 180g were randomly divided into four groups: control, L-NAME, FUPJ only, L-NAME+FUPJ. L-NAME was administered orally to the respective groups for 2 weeks, while FUPJ was given concurrently. Heart function parameters, including serum levels of creatine kinase (CK) and Lactate dehydrogenase (LDH) Were assessed. Histopathological examination and antioxidant enzyme activities were also evaluated. The result obtained showed that exposure to L-Name caused significant (p<0.05) increases in the activities of CK(58.87±0.04), LDH(40.16±0.18), T.C (176.31±0.27), and TAG (7.88±0.05),when compared to the normal control group. However, treatment with Fermented unripe plantain juice showed reduction in serum LDH (20.36±0.50) and CK (37.52±0.20) which attenuated L-Name induced cardiotoxicity in the rats. The decrease in the biochemical parameters such as LDH, CK, MDA, CAT, SOD, GSH observed in the L-Name administered rats were reversed in the rats administered with fermented unripe plantain juice. The antioxidant levels of SOD (3.98±0.18), CAT (2.13±0.04), and GSH (3.47±0.27) activities were considerably reduced (P<0.05), while MDA (5.23±0.05) concentration increased significantly after induction of L-Name. These conditions were, however, reversed by the administration of the unripe plantain juice. This result suggest that may have a protective effect on cardiotoxicity induced by L-name in rats.

Keywords: Cardiotoxicity Heart function L-NAME (N?-nitro-L-arginine methyl ester) fermented Histopathological.