CELL VIABILITY AND ANTITUMOR ACTIVITY OF PLANT PHENOLICS
Katerina Rouptiou, Rigini Papi, Amalia Aggeli and Maria Liakopoulou-Kyriakides*
ABSTRACT
Antioxidants protect cells from the damage caused by free radicals that are constantly generated in the human body, damaging tissues and leading to various diseases. Studies have shown that flavonoids reduce the risk of cardiovascular diseases, metabolic disorders, and other related diseases by reducing aggregation, acting as vasodilators in blood vessels. Here, extracts with major phenolics from the fruits of Lycium barbarum and Hippophae rhamnoides, Punika granatum reeds and wine marc as well as some pure phenolic acids and flavonoles were examined for their toxicity on mouse fibroblast cells L929 and two cancer cell lines, HeLa and MCF-7. Extracts at concentrations from 10 to 100 μg/ml of total phenolics were tested against the three cell lines using the MTT method. It was found that Goji berry and sea buckthorn phenolics affected cell proliferation of both HeLa and MCF-7 cell. Decrease on MCF-7 cell viability recorded by pomegranate while wine marc affected viability of HeLa cells. Possible apoptotic effect on HeLa and MCF-7 cells was also investigated using the multi-analyte profiling (xMAP) technology from Luminex.
Keywords: Punika granatum, Lycium barbarum, Hippophae rhamnoides, wine marc, phenolic compounds, cell survival, L929, HeLa, MCF-7 cells, apoptosis.
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