European Journal of Biomedical and Pharmaceutical Sciences

BIOSYNTHESIS OF SILVER NANOPARTICLES FROM ENTEROBACTER CLOACAE, RAOULTELLA TERRIGENA AND SOME OTHER ENTEROBACTERIA AND THEIR ANTIBACTERIAL ACTIVITY.

Layla A. H. Said*, Khawlah Jebur Khalaf and Manal Khalid Muhammad

ABSTRACT

This study aims to synthesize, characterize silver nanoparticles (AgNPs) using a cell free extract of some local clinical isolates belonging to Enterobacteriaceae namely: Enterobacter cloacae (EC), Enterobacter sakasakii (ES), Klebsiella pneumoniae (KP), Proteus mirabilis (PM) and Raoultella terrigena (RT). The obtained AgNPs were thoroughly analyzed using Atomic Force Microscopy (AFM) and the antibacterial activity of AgNPs produced was evaluated against some local pathogenic bacterial isolates using well diffusion method. AgNPs were produced by all isolates under study and their sizes were approximately in range from 79.45 to 102.25nm. All the synthesized AgNPs proved excellent antibacterial activity with zone of inhibition ranged between 12 to 23 mm. To the best of our knowledge, this is the first report of synthesis of AgNPs by Enterobacter cloacae and Raoultella terrigena species, and we believe that AgNPs may be considered a potential source of novel antibacterial agent against the emerging antimicrobial resistant pathogenic bacteria.

Keywords: silver nanoparticles, Enterobacter cloacae, Raoultella terrigena, bactericidal.