DELINEATING PHENOL DEGRADING BACTERIA FROM COIR RETTING CONTAMINATED SOILS OF SOUTHERN REGIONS OF CHERTHALA
Anoop M.* and Gayathri Premkumar
ABSTRACT
The coir retting industries in Southern regions of Cherthala provides a combative environment for the multiplication of specialized microbes with high phenol biodegradation potential. The present study aimed at isolation and characterization of phenol degrading bacteria from coir retting contaminated soils. After a series of screening enrichment techniques in MSPM at 37ºC, two bacteria were found to have the highest efficiency of pronounced growth in phenol stress conditions. Also, these bacteria have shown to be able to remove 22.5μM of phenol concentration that was measured by modified Mordaque et al, 1999 protocol at 24, 48 and 72 hours of incubation with a regular interval of 24 hours . The strong phenol degrading bacterial isolates were identified and the sequences were deposited in genbank as Lysini bacillus sp strain GAKVM (MT476862.1) and Paenibacillus sp strain. GA2KVM (MT509865.1). In the concentrations of 22.5μM of phenol, MT476862.1was able to degrade 74.44% and MT509865.1were able to degrade 74.77% within 72 hours of incubation at 37ºC.
Keywords: Coir retting, Biodegradation, Enrichment techniques, MSPM.
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