European Journal of Biomedical and Pharmaceutical Sciences

INVESTIGATE THE BIOACTIVE SPECTRUM OF THREE VARIOUS PLANT EXTRACTS AGAINST WOUND CAUSING DIABETIC PATHOGENS

Andrewslin Jani, V. and Anusha. S

ABSTRACT

Medicinal plants are a rich source of antimicrobial agents and provide a safer and cost effective way of treating infections against the pathogenic microbes. The present study describes the phytochemical profile and antimicrobial activity of three different medicinal plants such as Andrographis paniculata, Senna auriculata and Stevia rebaudiana along with three various solvents such as Acetone, Methanol. Ethanol, Chloroform and Control. Amoung the three plant, S. auriculata possessed widely seven phytochemical compounds such as Flavonoids, Tannins, Saponins, phenols, glycosides steroids and Coumarins and etc, In addition A. paniculata and S. rebaudiana extract showed only some phytochemical compounds such as four and five respectively. Antibacterial Spectrum: Besides, the antibacterial spectrum study made on five various diabetic wound pathogenic organisms; initially minimum (3±1.14 for Pseudomonas, 3±0.67 for E.coli and 3±1.03 for Streptococcus) to maximum (14±3.03 for E. coli) zone of inhibition was noticed on Choloroform and Acetone extract. It was the significant effect of the antibacterial activity against the wound pathogen. The second Plant extracts of S. auriculata showed a significantly immensed activity against Pseudomonas in acetone extract (8±0.45mm) also the similar effect has been noticed on methanol extract against the both organisms of Streptococcus (8±2.34) and Klebsiella (8±2.05) respectively. Moreover, the third plant such as S. rebaudiana showed a significantly maximum activity against Pseudomonas in acetone extract (18±2.30mm) it was one fold higher activity when compared with its control. Meanwhile, the highest concentration of 100μl also been given the least activity in chloroform extract (8±1.36) noted against the diabetic wound pathogenic organism of Pseudomonas. GC-MS Analysis: The methanolic extract of S. rebaudiana plant was subjected to GC-MS analysis and the meticulous compounds elucidation such as the active principle, area of the peak, Concentration (%) and Retention Time (RT) are presented in present on table-5. Initially the two identical prevailing peak compounds were observed such as 1-Naphthalenepropanol alpha.-ethenylde1-and Naphthalenepentanol, decahydro-5- (hydr) and its representing abundance is similar range 525% but retention time was varied from 32.7 to 34.19. Moreover, other second peak compound was 3, 7, 11, 15-Tetramethyl-2- hexadecen-1-ol noted at 24.0 mints of retention time with 410% of abundance. From the present result clearly indicated that these three plant extracts are act as a excellent antibacterial activity against the diabetic wound pathogenic organism.

Keywords: Andrographis paniculata, Senna auriculata and Stevia rebaudiana, diabetic wound pathogenic.