European Journal of Biomedical and Pharmaceutical Sciences

ANTIOXIDANT AND HYPOLIPIDEMIC EFFECTS OF METHANOL FRACTION OF MANGIFERA HADEN SEEDS ON ALBINO RATS INDUCED HYPERLIPIDEMIC RATS

Maryann Nonye Nwafor*, Onyinyechi Cynthia Onyekwere, Chinedu Shedrack Ogunwa,
Michael Obinna Eji, Chinemelum Adaora Onodugo, Ikimi Charles German, Abba Babandi and
Whyte Jim Alalibo

ABSTRACT

Antioxidants are substances which inhibit oxidative damage by trapping free radicals to a target molecule. They are quite necessary to overcome the chronic metabolic disorder characterized by elevated circulating levels of lipids. The present study investigated the antioxidant and hypolipidemic potentials of Mangifera haden seeds in carbon tetrachloride induced hyperlipidemic rats. The animals were acclimatized to the laboratory conditions for a period of seven days. At the end of the acclimatization period, each rat was weighed and randomly divided into six (6) groups of five animals each, comprising of test animals and control groups. Proximate analysis, antioxidant activity, toxicity studies and lipid profile parameters were determined in the study using standard methods. Proximate composition of whole sample of Mangifera haden seeds revealed that the (%) values of protein, lipid, crude fibre, ash, moisture, and carbohydrate are (4.82, 17.18, 4.29, 2.77, 8.39, 62.55) respectively. The fraction of Mangifera haden seeds were shown to have high antioxidant activity. All carbon tetrachloride induced animals displayed hyperlipidemia as shown by their elevated levels of serum total cholesterol (TC), triacylglycerol (TAG), low density lipoprotein (LDL-c), very low density lipoprotein (VLDL-c) and reduction in high density lipoprotein (HDL-c). Methanol fraction of Mangifera haden seeds were administered at different doses (200 mg, 400 mg, 600 mg) of the fraction per kilogram body weight of the animals in carbon tetrachloride hyperlipidemic rats. Simvastatin was used as reference standards. Simvastatin was found to be an effective drug in lowering lipid profiles. The statistical analyses were carried out using one way ANOVA followed by Dunnett’s Post Hoc Multiple Comparisons test. Mangifera haden seeds show significant decrease (P < 0.05) in the levels of serum Total Cholesterol, Triacylglycerols, LDL-C, VLDL-C, Atherogenic index (A.I) and significant increase (P < 0.05) of serum HDL-C against carbon tetrachloride-induced hyperlipidemic rats. The result also suggest that at 200 mg/kg Mangifera haden body weight concentrations are an excellent lipid lowering agent. Mangifera haden seeds exhibited quite hypolipidemic potential when compared with the reference drug, simvastatin. This indicates that the organic extracts could be explored as an alternative therapeutic agent in the treatment of hyperlipidemia, obesity and abdominal fats.

Keywords: Antioxidant, Mangifera haden, hyperlipidemia, atherogenic index, and simvastatin.