Abstract
DEVELOPMENT AND VALIDATION OF UV SPECTROSCOPIC METHOD FOR ESTIMATION OF CIDOFOVIR DIHYDRATE IN CIDOFOVIR INJECTION

Kalyani S. Shewale*, Shruti D. Pawar, Manudip S. Deore, Sanket A. Bagad, Rakesh S. Ahire and Nishant B. Chinchore

ABSTRACT

Objective: To develop and validate simple, rapid, linear, accurate, precise and economical UV Spectroscopic method for estimation of Cidofovir Dihydrate in Cidofovir Injection form. Methods: The drug is soluble in 0.1 N Sodium Hydroxide. The drug was identified in terms of solubility studies and on the basis of melting point done on melting point apparatus of Equiptronics. It showed absorption maxima were determined in 0.1 N Sodium Hydroxide. The drug obeyed the Beer’s law and showed good correlation of concentration with absorption which reflect in linearity. The UV spectroscopic method was developed for estimation of Cidofovir in injection form and also validated as per ICH guidelines. Results: The drug is freely soluble in 0.1 N Sodium Hydroxide, sparingly soluble in Methanol, Ethanol and insoluble in Chloroform. So, the 0.1 N Sodium Hydroxide is used as a diluent in method. The melting point of Cidofovir was found to be 258-259˚C (uncorrected). It showed absorption maxima 290 nm in 0.1 N Sodium Hydroxide. On the basis of absorption spectrum the working concentration was set on 30 μg/ml (PPM). The linearity was observed between 10-50 μg/ml (PPM). The results of analysis were validated by recovery studies. The recovery was found to be 100, 99 and 98.6% for three levels respectively. The % RSD for precision and ruggedness was found to be 0.82% and 0.19% respectively. Conclusion: A simple, rapid, linear, accurate, precise and economical UV Spectroscopic method has been developed for estimation of Cidofovir in tablet dosage form. The method could be considered for the determination of Cidofovir in quality control laboratories.

Keywords: Cidofovir, UV Spectrophotometer, Melting Point, Assay Method, Validation, Accuracy, Linearity, Ruggedness, Precision.


[Full Text Article]   [Download Certificate]

Indexing

Indexing

Best Article Awards

European Journal of Biomedical and Pharmaceutical Sciences (EJBPS) is giving Best Article Award in every Issue for Best Article and Issue Certificate of Appreciation to the Authors to promote research activity of scholar.

Best Article of current issue :

Dr. Dhrubo Jyoti Sen

Download Article : Click here

News & Updation

  • EJBPS: New Impact Factor 2026

    EJBPS Impact Factor has been Increased from  7.482 to 8.181 for Year 2026.

  • EJBPS: FEBRUARY ISSUE PUBLISHED

    FEBRUARY 2026 Issue has been successfully launched on 1 FEBRUARY 2026.

  • Index Copernicus Value

    EJBPS Received Index Copernicus Value 77.3, due to High Quality Publication in EJBPS at International Level

  • Journal web site support Internet Explorer, Google Chrome, Mozilla Firefox, Opera, Saffari for easy download of article without any trouble.

    .

  • Article Invited for Publication

    Dear Researcher, Article Invited for Publication  in EJBPS coming Issue.

     
Google Scholar Index Copernicus Indian Science Publications Urlich's Periodicals Directory, Proquest, UK (In Process) Research Bible, Fuchu, Tokyo. JAPAN UDLedge Science Citation Index International Society for Research activity (ISRA) Scientific Indexing Services (SIS) InfoBase Index (In Process) SJIF Impact Factor Scholar Article Impact Factor, SAIF Universal Impact Factor CAS (A Division of American Chemical Society) USA (Under Process) Directory of Open Access Journal (DOAJ, Sweden, in process) CiteFactor Directory Of Research Journal Indexing (DRJI) Indian citation Index (ICI) Journal Index (JI, Under Process) Directory of abstract indexing for Journals (DAIJ) Open Access Journals (Under Process) Impact Factor Services For International Journals (IFSIJ) Cosmos Impact Factor Jour Informatics (Under Process) Eurasian Scientific Journal Index (ESJI) International Innovative Journal Impact Factor (IIJIF) Science Library Index, Dubai, United Arab Emirates Global Impact Factor (GIF) (0.377) IP Indexing (IP Value 3.77) Web of Science Group (Under Process) Directory of Research Journals Indexing Scope Database Academia Academia Doi-Digital Online Identifier Doi-Digital Online Identifier ISSN National Centre Zenodo Indexing International CODEN Service, USA

UG/PG/Ph.D Research Publication

Research Scholar of UG/PG/Ph.D can Submit their Research Article/Review Article/Case Study/Short Communication for Publication in EJBPS

Downloads

Copyright From

Covering Letter

                        Author Instruction 
 

PLAGERLUM REPORT