European Journal of Biomedical and Pharmaceutical Sciences

EVALUATION OF HORMONAL LEVEL AND HISTOMORPHOLOGICAL CHANGES IN LEAD ACETATE INDUCED MALE ALBINO RATS TREATED WITH ALLIUM CEPA AND CURCUMA LONGA ETHANOIC EXTRACTS IN MANAGEMENT OF TESTICULAR DAMAGE

*Chiamaka Onyedika Chukwuma, Prudence N. Okara and Chidimma Anthonia Azike

ABSTRACT

Lead poisoning has been implicated in the pathogenesis of several diseases. This study evaluated hormonal levels and histomorphological changes in male albino rats induced with lead acetate and treated with Allium cepa and Curcuma longa ethanoic extracts in management of testicular damage. This study was conducted on 35 male albino rats divided into 5 treatment groups; Group 1 (Negative control), Group 2 (positive control), Group 3 (treated with Allium cepa only), Group 4 (treated with Curcuma longa only) Group 5(treated with combination of Allium cepa and Curcuma longa extracts) for 4 weeks before sacrificing and harvesting the testes for histological analysis, and blood sample obtained through cardiac puncture for hormonal analysis. Group 1-5 were induced with 100mg/kg body weight of lead acetate. Using the Layman L.C method, Follicle Stimulating Hormone and Luteinizing hormone levels were estimated. There were significant increases in the levels of FSH (P=<0.001) and LH (P=0.010) in the treatment groups compared to Group 2 (positive control). This indicates the ability of the extracts to attenuate hormonal imbalance. Thus, the Allium cepa and Curcuma longa ethanoic extracts can be used as a safe therapy for the management of hormonal imbalance resulting from testicular damage.

Keywords: Hormonal Level, Histomorphology, Lead Acetate, Allium cepa, Curcuma longa, Testicular damage.