European Journal of Biomedical and Pharmaceutical Sciences

STUDY ON DECELLULARIZING PORCINE VESSEL FOR MAKING ARTIFICIAL VASCULAR GRAFT

Quan Minh To* and Ha Le Bao Tran

ABSTRACT

In 2011, cardiovascular disease (CVD) still accounted for 31.3% of all deaths in America. One-third of the patients who need an arterial bypass procedure don’t have suitable autologous vessels. Decellularization of allogeneic/xenogeneic vessels can bring new hope for patients. Porcine coronary arteries were bought from the market. They were treated with 1M NaOH for 6h or 0.1% Triton X100 for 24h or distilled water for 24h or 0.5% SDS for 24h or a combination of 0.5% SDS for 24 hours and distilled water for 24 hours. Decellularization efficiency was determined by HE staining and Trichrome staining. Acellular vascular grafts were disinfected by 0.15% glutaraldehyde for 1 hour. In vitro cytotoxicity of acellular vessels was tested by direct contact method according to ISO 10993-5. The umbilical cord blood-derived endothelial progenitor cells were seeded on lumen of acellular arterial pieces with concentration of 5x103 cells/piece and cultured for seven days. The growth of UCB EPC was tested by MTT assay, HE staining and SEM. Result: the combination of SDS 24h and distilled water 24h is the most efficient method in this study: cells were completely removed and extracellular matrix (ECM) was preserved. GA-treated acellular vessel was not toxic to fibroblast in vitro, according to ISO 10993-5. UCB-EPC grew on lumen of acellular arterial pieces from day 1 to day 7. Conclusion: decellularized vascular grafts were successfully made from porcine vessel by SDS and distilled water. GA can be used for disinfectant and not harmful in vitro. UCB EPC can grow on lumen of acellular vessels.

Keywords: Decellularization, cardiovascular disease, porcine coronary artery, vascular tissue engineering, endothelial progenitor cell.