MOLLUSCICIDAL EFFECT OF BINARY COMBINATIONS [1:1] OF AQUEOUS STEM BARK EXTRACT WITH ACTIVE CONSTITUENTS OF EUPHORBIA TIRUCALLI ON FRESHWATER SNAIL INDOPLANORBIS EXUSTUS
Reshmina Firoz Khan* and Ram P. Yadav
ABSTRACT
The binary combinations of the aqueous extract of stem bark and the active moieties of the plant Euphorbia
tirucalli were tested against freshwater vector snail Indoplanorbis exustus for their molluscicidal activity.
Accordingly, binary (1:1) combinations of the gallic acid and quercetin with aqueous stem bark extract of
Euphorbia tirucalli, were studied for their toxic activity on the vector snail. The result was observed that
molluscicidal activity of Euphorbia tirucalli aqueous stem bark with the other two active constituents against
freshwater vector snail Indoplanorbis exustus was both time as well as dose dependent. A significant negative
correlation was observed between the LC50 values and exposure periods, thus the LC50 values in binary (1:1)
combinations of the gallic acid and quercetin with aqueous stem bark extract of Euphorbia tirucalli decreased
from 6.28 mg/L (24h) to 3.79 mg/L (96h) and from 4.90 mg/L (24h) to 1.71 mg/L (96h) respectively, against the
vector snail Indoplanorbis exustus. The toxicity test of binary combinations showed that the combination of active
compound quercetin with stem bark of Euphorbia tirucalli was more toxic than the binary combination of active
compound gallic acid with the stem bark of Euphorbia tirucalli. Thus, it can be concluded that the above binary
combinations can be used as more effective and potent molluscicides.
Keywords: Molluscicides, Euphorbia tirucalli, quercetin, gallic acid, Indoplankrbis exustus.
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