European Journal of Biomedical and Pharmaceutical Sciences

FORMULATION AND DEVELOPMENT OF MICROSPHERES LOADED GEL OF ANNONA SQUAMOSA EXTRACT FOR POTENTIAL ANTIMICROBIAL ACTIVITY

Ansari Kaushar, Dhote Vinod*, Dhote Kanika and Jain Surendra

ABSTRACT

Microspheres are small spherical Particles, with a diameter in the micrometer Range (typically 1 micrometre to 1000) micrometer Microspheres are sometimes referred to as microparticles. Microspheres can be manufactured from various natural and synthetic materials. Glass microspheres, polymer microspheres, and ceramic microspheres are commercially available Solid and Hollow microspheres Vary widely in Density and therefore, are used for different applications. Qualitative phytochemical screening of Annona squamosa showed the presence of active metabolites such as Alkaloids, flavonoids, glycosides, steroids, tannin and phenols is present. Formulation was carried out by Solvent Evaporation method. Trial batches indicated that polymers are suitable for the Annona squamosa microspheres. Polymer produced good formulations. HPMC and ethyl cellulose were selected for further studies. Scanning electron micrograph of the prepared microspheres at 1.00 kx magnification showed that the microspheres were smooth surface morphology and spherical shape. The spongy and porous nature of microspheres was clearly observed in the SEM images. Particle size and zeta potential was determined by Malvern Zeta sizer. The particle size analysis confirmed that the prepared sample were in the nanometer range. Average particle size obtained for the formulations F1 and F5 were 143.0 to 171.5 nm. Zeta potential values of microspheres indicated that the formulated microspheres are stable. The amount of drug being entrapped in microspheres was calculated and all the prepared microspheres were found to possess very high entrapment efficiency.

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