European Journal of Biomedical and Pharmaceutical Sciences

EXTRACTION AND ISOLATION OF CARPAINE FROM CARICA PAPAYA LEAF

Tiwari Nandita*, Khan Sahabulla, Agrawal Ankita and Dr. Hitesh Dalvadi

ABSTRACT

There have been numerous studies conducted on humans and animal models to confirm the effectiveness of papaya leaves extract for the treatment of thrombocytopenia. In the present study, the alkaloid, carpaine extracted from papaya leaves was found to have platelet stimulating activity. Carpaine is isolated from the papaya leaf extract by using the Soxhlet extraction method. For extraction 64 g of leaves powder was extracted with a suitable mixture of solvents like Methanol, Ethanol, Glacial acetic acid, HCL, and Water in Soxhlet apparatus for 48 h at 70°. Then the Carpaine should be isolated from the papaya leaf extract by treating the extract with the solvent like petroleum ether, ammonia, chloroform, n-hexane. Carpaine present in papaya leaf shows Anti-thrombocytopenic activity. The isolated Carpaine has been evaluated for the various evaluation parameters such as Phytochemical, TLC, HPTLC, FTIR, and DSC analysis. The result of preliminary phytochemical test screening ofextract of carica papaya leaf powder revealed that presence of alkaloids. Thin layer chromatography studies of this compound were optimized with the solvent system which showed a spot at RF = 0.74. In HPTLC the RF value of Carpaine was found to be 0.94. DSC the thermogram obtained having two peaks at 133.22 and 242.56°C respectively. In FTIR the presence of functional groups was confirmed and matched with standard range and observed value. From above result, It is concluded Carpaine was Extracted and Isolated from papaya leaf.

Keywords: Carica Papaya Leaf, Anti-thrombocytopenia, Carpaine, Extraction, Isolation, HPTLC, DSC, FTIR.