European Journal of Biomedical and Pharmaceutical Sciences

RP-HPLC ANALYTICAL METHOD DEVELOPMENT AND VALIDATION FOR ETORICOXIB AND TRAMADOL IN SYNTHETIC MIXTURE

Bhakti Dave, Chainesh Shah*, Komal Chaudhary, Sharma Sheetal, Priyanka Patil, Umesh M. Upadhyay

ABSTRACT

A Specific, precise, accurate, Robust and cost-effective RP-HPLC method was developed for the Simultaneous
Estimation of Etoricoxib and Tramadol in Synthetic Mixture. RP-HPLC method was designed using an HPLC
system. After optimization of good chromatographic separation was achieved with a mixture of ortho-phosphoric
acid (OPA), methanol, and acetonitrile were filled in different mobile phase reservoir after filter and sonicate to
degas the mixture. Mobile phase Buffer (pH 5 adjusted with OPA): methanol, and acetonitrile in the volume ratio
30:35:35v/v/v were used as the mobile phase with, Shimpack ODS C18 column 25 cm (4.6 mm x 250mm, 5 um)
as stationary phase at flow rate of 1 mL/min and detection wavelength of 220 nm. The retention time of Etoricoxib
and Tramadol was found to be 5.092 min and 14.30 min respectively.

Keywords: RP-HPLC, Etoricoxib, Tramadol, orthophosphoric acid, RSD.