IN VITRO EVALUATION OF CYTOTOXIC ACTIVITY OF PIPER LONGUM L. ROOT (LONG PEPPER) ON SOME IMPORTANT SPECIES OF BACTERIA
Kiran B.*, Sakchi Bhushan and Lalitha V.
ABSTRACT
In vitro evaluation of antibacterial activity of aqueous and solvent extract of Piper longum root was evaluated against five bacterial species viz., Staphylococcus aureus Escherichia coli, Bacillus subtilis, Klebsiella pneumonia and Pseudomonas aeruginosa. In aqueous extract tested at 10 to 100% concentration, maximum inhibition was observed in S. aureus (31.0mm) and B. subtilis (31.0mm) followed by E. coli (30.0mm) and P. aeruginosa (23.0mm). In 10 to 90% concentration, the inhibition percentage was in the range of 5.0 to 29.0 mm respectively. In solvent extract, high percentage of inhibition was observed in petroleum ether extract and recorded a maximum inhibition of 32.0mm and minimum of 27.0mm in all test bacterial species. Petroleum ether extract was followed by methanol, chloroform and ethanol extract. Compared to synthetic antibiotic xanthomycein, chloramphenicol and streptomycein at 25mg concentration, maximum inhibition of 35.0 mm and minimum of 30.0mm was observed in all the test bacteria. The Minimum Inhibitory Concentration (MIC) was identified for all the test bacterial species.
Keywords: Bacteria, Aqueous extract, Solvent extract, MIC, Synthetic antibiotic.
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